Sorority Row is a story about a college prank gone bad. To get back at her boyfriend Garret for cheating on her, Megan talks her sorority sisters, Cassidy, Ellie, Jessica, Claire, and Chugs into helping her trick Garret into believing she is dead. They fake her death and go to an abandoned steel mill to dispose of her. Garret starts freaking out and stabs Megan with a tire iron, actually killing her. The girls discuss whether or not to call the police, but they decide to just dump Megan's body, agreeing to never speak of it again.
Eight months later at the girl's graduation party Ellie thinks she sees Megan, but it turned out to actually be Megan's sister. Then the sisters receive a picture message of a someone holding a bloody tire iron. Panick-ing, the girls try to figure out who is doing this. The picture leads them to believe it is Garret and he's seeking his revenge now.
Chugs is the first to be killed. Later, Claire breaks up with her boyfriend Mickey, who later tries to kiss Ellie. When she refused he wondered off into the attic where the killer made Mickey his second victim. Ellie find his body and goes running to tell her sisters. The girls go back to the steel mill after getting another message, this time a video of them killing Megan. They find Garret there trying to commit suicide, but the girls take action and run him over.
They then return to the party to find everyone gone. After killing Jessica and Claire, the killer is face to face with Cassidy. She finds out it was her boyfriend Andy the whole time and as he was about to kill her, Ellie comes in and shoots him. Fifteen months later the sorority house is under renovation and Maggie, Megan's sister, is now a Theta Pi sister just like she was.