Space Buddies is a children's comedy film and the third installment in the Air Buddies series. It was released direct-to-DVD and Blu-ray in 2009. When Space Buddies begins, Buddha and his owner, Sam, are looking up at the stars. A shooting star speeds across the sky, and Sam wishes he could touch the moon. Sam takes a field trip with his classmates the following day to Vision Enterprises to observe a test launch of a spacecraft called Vision 1. Since animals are not allowed on the trip, Sam leaves the puppy at home. Buddha gets together with his fellow Buddies, Rosebud, Mudblud, B-Dawg and Budderball, and convinces them to join him to watch the launch.
When they arrive at Vision Enterprises, the puppies go inside a spacesuit machine and emerge wearing spacesuits. They follow the students to the spacecraft and climb aboard. Buddha and the rest of the Buddies spend time investigating the shuttle, but they soon find themselves sealed inside the craft with pilot Astro, and the shuttle launches into outer space.
Vision 1 is running low on fuel, so Mission Control sends the shuttle to an old research space station to refuel. When they arrive, Buddha and the others explore the space station, where they meet a dog named Spudnick, who is owned by Yuri, an astronaut living in the space station. When Yuri meets the puppies, he is happy to have them as company, but tries to trap them inside the space station. Spudnick and the Buddies ultimately escape, as does Yuri.
The shuttle then travels to the moon. Mission Control determines that the dogs are in space, and the news is broadcast on television for the children to see. The Buddies travel through a meteor shower as they embark on a dangerous voyage back to Earth.
When they land, the Buddies are rewarded for their bravery and honored with the title of "Space Buddies". Buddha gives Sam a rock he found on the moon, allowing Sam's wish of touching the moon to come true.